1. It is given a sharp knock to the pot that CESIRE has given us so that the flies go to the bottom of the pot.

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2. A previously wet cotton plug with ether is placed inside the pot that we want to take the flies, it is left for 15-20 seconds since they will die instead of falling asleep.

 

 

 

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3. After removing the ether cap, the cap is placed without ether so as not to kill the individuals.

4. The sex is determined and those flies that are wanted to study are placed in the definitive pot.

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5. Let's cover the flies that are found in the final bottle with the caps made of crumb and cotton.

6. Once we have done this process with the ten bottles, the crossings that have been carried out and the number of the replica must be labeled specifically.

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